Sunihapsari, Cici (2012) Hubungan Mutasi Gen p53 Penderita Karsinoma Nasofaring WHO Tipe III Stadium Lanjut dengan Riwayat Merokok. Magister thesis, Universitas Brawijaya.
Abstract
Karsinoma nasofaring (KNF) adalah keganasan dengan etiologi multifaktorial yaitu infeksi virus Epstein Barr , faktor genetik dan faktor lingkungan. Faktor lingkungan yang mengandung bahan karsinogen salah satu diantaranya adalah asap rokok. Gen p53 adalah salah satu tumor suppressor gen yang memiliki peran pada proses karsinogenesis. Mutasi gen p53 sering ditemukan pada keganasan di manusia. Mutasi gen p53 menyebabkan apoptosis dan penuaan seluler menurun serta siklus sel tidak berhenti. Penelitian ini bertujuan untuk mengetahui hubungan mutasi gen p53 pada penderita KNF WHO tipe III stadium lanjut dengan riwayat merokok (jumlah rokok batang per hari, lama merokok, dan paparan kumulatif rokok). Penelitian ini menggunakan metode cross sectional . Subyek penelitian sejumlah 30 penderita KNF WHO tipe III stadium lanjut (didiagnosis di poli THT RSUD dr.Saiful Anwar Malang, sejak Oktober 2011 sampai Mei 2012) yang diambil jaringan biopsy nasofaringnya. Pemeriksaan mutasi gen p53 diketahui dari pemeriksaan PCR dan RFLP. Analisa statistik menggunakan uji Fisher `s Exact dan uji T. Dari 30 subyek penelitian, hanya 29 subyek penelitian yang dapat diketahui ada tidaknya mutasi gen p53. Sebanyak 27 (93.1%) subyek penelitian mengalami mutasi gen p53 (17 subyek penelitian dengan riwayat merokok dan 10 subyek penelitian tanpa riwayat merokok). Dua subyek penelitian lainnya (6.9%) tidak mengalami mutasi gen p53 dengan masing-masing 1 subyek penelitian dengan dan tanpa riwayat merokok.Hasil uji Fisher `s Exact tidak menunjukkan hubungan signifikan antara riwayat merokok dengan terjadinya mutasi gen p53 (p-1.000). Hasil uji T mutasi gen p53 terhadap jumlah rokok per hari (p=0.981), lama merokok (p=0.613) dan paparan kumulatif merokok (p=0.864) tidak menunjukkan adanya hubungan yang signifikan. Kesimpulan: mutasi gen p53 ditemukan terjadi pada 93.1% penderita KNF WHO tipe III stadium lanjut. Mutasi gen p53 dengan riwayat merokok (jumlah rokok per hari, lama merokok dan paparan kumulatif) tidak menunjukkan adanya hubungan yang signifikan.
English Abstract
Nasopharyngeal carcinoma (NPC) is a malignancy with multifactorial etiology involving Epstein Barr virus infection, genetic and environmental factors. Cigarette smoking is one of environmental factor which has many compounds of carcinogen. p53 gene as a tumor suppressor plays an important role in carcinogenesis. It is found mutated in almost of human malignancy. Mutation of p53 gene causes decrease of apoptosis and cellular senescence also failure of cycle cell arrest. This study was conducted to determine correlation between p53 gene mutation in end stage type III WHO NPC with cigarette smoking history (quantity, duration and cumulative exposure). Thirty patients who were diagnosed as end stage type III WHO NPC between 2011 October and 2012 May at Otorhinolaryngology Outpatient Departement of Saiful Anwar Hospital in Malang, were included in this cross sectional study. Thirty NPC biopsies were collected and assessed for p53 gene mutation by PCR and RFLP. Fisher`s exact test and T test were used to analyse correlation p53 gene mutation and cigarette smoking history. Among a total of 30 samples, 29 samples showed examination result of p53 gene mutation. mutation of p53 gene was observed in 27 (93.1%) consist of 17 subjects with cigarette smoking history and 10 subjects without cigarette smoking history. Two subjects (6.9%) showed no mutation p53 gene (each of se with and without cigarette smoking history). re was no significant correlation between cigarette smoking history and p53 gene mutation; showed by Fisher`s exact test (p=1.000). After statistical analysis according to T test, no significant correlation between p53 gene mutation with quantity (p=0.981), duration (p=0.613) and cumulative exposure of cigarette smoking(p=0.864). In conclusion, p53 gene mutation was found in 93.1% end stage type III NPC patients. p53 gene mutation was not correlated with quantity, duration and cumulative exposure of cigarette smoking.
| Item Type: | Thesis (Magister) |
|---|---|
| Identification Number: | TES/616.21/SUN/h/041204696 |
| Subjects: | 600 Technology (Applied sciences) > 616 Diseases > 616.2 Diseases of respiratory system |
| Divisions: | Profesi Kedokteran > Spesialis THT Kepala dan Leher, Fakultas Kedokteran |
| Depositing User: | Endro Setyobudi |
| Date Deposited: | 03 Jan 2013 08:58 |
| Last Modified: | 03 Jan 2013 08:58 |
| URI: | http://repository.ub.ac.id/id/eprint/158308 |
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