Dayeef, AzimYahiaM (2013) The Influnce Of Annona Muricata Leaves Extract In Damaging Kidney Cell, Increasing Creatinine Serum And Inducing Caspase-9 Activity. Magister thesis, Universitas Brawijaya.
Abstract
Dalam pengobatan tradisional Indonesia, ekstrak daun Annona muricata dipakai untuk mengobati berbagai macam penyakit. Beberapa penelitian terdahulu telah membuktikan bahwa Annona muricata efektif mengobati kerusakan hati yang disebabkan oleh karbon tetraklorida dan asetaminofen. Penelitian ini bertujuan untuk membuktikan keefektifan potensi sitotoksisitas ekstrak etanolik daun Annona muricata dan keefektifan ekstrak etanolik daun Annona muricata ini dalam menginduksi apoptosis pada sel tubular. Penapisan fitokimia menunjukkan adanya senyawa alkaloid, tanin, flavonoid, saponin, antrakuinon, dan kardiak glikosida. Penelitian ini dilakukan untuk mengetahui pengaruh ekstrak daun Annona muricata Linn pada tingkat serum kreatinin dan pada kerusakan struktur sel tubular yang mempengaruhi fungsi sel ginjal dan ekspresi caspase-9 pada sel glomerulus and tubular. Hewan coba (n=20) dikelompokkan menjadi 4 kelompok yang terdiri dari kelompok kontrol dan kelompok perlakuan I, II, and III. Hewan coba pada kelompok perlakuan I diberi 10 mg/kg ekstrak Annona muricata per bb/ hari yang direndam dalam larutan saline selama 40 hari, hewan coba pada kelompok II diberi 20 mg/kg ekstrak daun Annona muricata per bb/ hari yang direndam dalam larutan saline selama 40 hari dan kelompok III diberi 40 mg/kg ekstrak daun Annona muricata per bb/ hari yang direndam dalam larutan saline selama 40 hari. ELISA dipakai untuk mengetahui pengaruh ekstrak pada konsentrasi serum kreatinin. Glomerulus dan sel tubular cells dipersiapkan untuk melihat perubahan histologis dan ekspresi caspase-9.
English Abstract
Annona muricata leaf extract is used in Indonesia traditional medicine for treatment of many disease. Work done has demonstrated Annona muricata to be effective in treating carbon tetrachloride and acetaminophen induced hepatic damage, In this present study, efficacy of ethanolic extracts of Annona muricata leaves for its cytotoxicity potential and induction of apoptosis in tubular cells, was investigated. Phytochemical screening verified presence of alkaloids, tannins, flavonoids, saponins, anthraquinones and cardiac glycosides. present study was undertaken to investigate leaf extract effects of Annona muricata Linn. on level of serum creatinine and damage of tubular cell structure that effect of kidney cell functions and caspase-9 expression in glomerulus and tubular cells. animals (n=20) Were grouped a (control), I, II, and III (experimental). experimental animals of group I were administered with 10 mg/kg body weight/ day Annona muricata extract in saline for 40 days, group II were administered with 20 mg/kg body weight/ day Annona muricata extract in saline for 40 days and III were administered with 40 mg/kg body weight/day Annona muricata extract in saline for 40 days and n we see effect on creatinine serum concentration by ELISA method and glomerulus, tubular cells were fixed and processed to examine histological changes and caspase-9 e pression in glomerulus and tubular cells.
| Item Type: | Thesis (Magister) |
|---|---|
| Identification Number: | TES/615.324 39/DAY/t/041400554 |
| Subjects: | 600 Technology (Applied sciences) > 615 Pharmacology and therapeutics > 615.3 Organics drugs |
| Divisions: | S2/S3 > Magister Ilmu Biomedis, Fakultas Kedokteran |
| Depositing User: | Budi Wahyono Wahyono |
| Date Deposited: | 19 May 2014 08:20 |
| Last Modified: | 19 May 2014 08:20 |
| URI: | http://repository.ub.ac.id/id/eprint/158132 |
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