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Violandoa, Wiga Alif (2017) Study of Inorganic Arsenic Effect on Growth of Padina minor. Study of Inorganic Arsenic Effect on Growth of Padina minor.

Indonesian Abstract

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English Abstract

Omnipresent of arsenic (As), especially on aquatic ecosystem, had been treated healthy and quality of any organism like seaweed. Seaweed as passive aqua-plant in coastal zone had been reported with massive accumulation of every pollution from terrestrial activities. Brown seaweed such as Padina minor had a good ability to bind and accumulate any metal contaminants. However, the detoxification mechanism and antioxidant synthesize to defense against toxicity of As are less to observe. This research aimed to observe the As accumulation and detoxification time, also the antioxidant defense activities on P. minor. Each gram of P. minor was cultured in various As contamination media (As3+, As5+ and Asmix) over a period of 7 days, then reculture to As-free media for 14 days more to observe the detoxifying ability. Different impact on growth and accumulation of inorganic arsenic (iAs) and total arsenic (tAs) contents were showed after polluted with various iAs variant level. Negative growth value was occurred on all variant treatments, except 25 μg g-1 of mixed iAs at day 7. Interestingly, the thalli seems detoxified the toxicity of iAs after reculture in As-free media, but not after exposed with various As5+. Dying thali was indicates that As5+ had a lethal effect than As3+ on P. minor thali, even in low concentration. While on 25 μg g-1 of mixed iAs showed an acute effects on thali by decreased its growth slowly till final day used a small amount accumulation. Meanwhile, direct attenuation impact was accumulated massively at 50 and 100 μg iAs g-1. The thali started to recover after reculture in health environment for 14 days. Massive amount of As contents shown after 7 days culture in all As variant treatments. However, only As5+ was detected on the thali at day 7 and 21 which indicated internal oxidation of As3+ was done before 7 days. Highest accumulation of As5+ was found at day 7 after exposed with 100 μg g-1 As5+ while 25 μg g-1 Asmix at day 21 as the fewest one. Various antioxidant activities, such as decreasing polysaccharide, increasing activities of DPPH and flavonoid, indicated that iAs was intruded and accumulated inside the cell directly. Those parameters showed the decreasing activities at 100 μg iAs g-1, indicated that 50 μg iAs g-1 was an optimum iAs level on P. minor. Meanwhile, iron chelation activity was relatively low and stable weakened by iAs toxicity since day 7. The results indicate that P. minor has ability to oxidase and methylate with antioxidant role as defensive agent against iAs, but it also needs more than 14 days to recover in high level contamination

Other Language Abstract

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Item Type: Article
Identification Number: PUB/577.627/VIO/s/2017/081706897
Uncontrolled Keywords: ARSENIC - TOXICOLOGY, ARSENIC - ENVIRONMENT ASPECTS, ARSENIC - BIOACCUMULATION, WATER - POLLUTION, MARINE ALGAE, MARINE ALGAE - TOXICOLOGY
Subjects: 500 Natural sciences and mathematics > 577 Ecology > 577.6 Aquatic ecology > 577.627 Water Pollution (Effect on natural ecology)
Divisions: S2 / S3 > Magister Budidaya Perairan, Fakultas Perikanan dan Ilmu Kelautan
Depositing User: Nur Cholis
URI: http://repository.ub.ac.id/id/eprint/1441
Text
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