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Violando, Wiga Alif (2017) Study on Inorganic Arsenic Effect on Growth of Padina minor. Magister thesis, Universitas Brawijaya.

Indonesian Abstract

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English Abstract

High activity of human and industry were makes much problem by its pollution on environment. Heavy metal was one of the substantial pollution by industrial activity that difficult to degrade in nature. Arsenic is one of the most dangerous heavy metal pollution. It was difficult to detect, long time to detoxified, and extremely poisonous for living organism. This study was conduct to reveal the ability of brown seaweed Padina minor to absorb, accumulate, and recover after exposure with inorganic arsenic. The seaweeds were culture in inorganic arsenic-contaminated environment with different concentration (25 ppb, 50 ppb, and 100 ppb) for 7 days than moved to arsenic-free environment till the 21 days. The arsenic intakes were observed for 7 days and 21 days, while growth rates were measured for each week. The inorganic arsenic contents including arsenite (As3+) and arsenate (As5+) were measured by ICP-MS, while total arsenic contents were performed by AAS. Antioxidant activities have been examined to verify the condition of samples on day 7 and day 21. iv All of thalli were displayed in negative growth rate after been treated with various concentrations of As3+ and As5+ addition. However, thalli showed positive growth rate once removed from As3+ treatments, but not for As5+ treatments. Higher As5+ addition resulted in lower growth rates even thalli were put into As5+-free condition after 14 days. In mixed arsenic treatments, thalli displayed positive growth after removed from arsenic-free condition for 14 days. Thereafter, the results suggested As5+ may be more toxic than As3+ for P. minor. Interestingly, there were not found As3+ form inside seaweed on day 7 neither nor on day 21, however, it was found only as As5+ form by the results. There were found around 4.57 μg As5+ g-1 on 25 ppb of As3+ and it were increased by elevation of As3+ concentration until 10.94 μg As5+ g-1 on 100 ppb of inducing As3+, even only 10% of As3+ was absorb by the seaweeds after 7 days. While around 2.8 μg As5+ g-1 on 25 ppb of As5+ and it increased by elevation of As5+ concentration until reach 41.89 μg As5+ g-1 on 100 ppb treatment. On 25 ppb of mixed inorganic (As3+ and As5+) were found 2.29 μg As5+ g-1 and reach to 14.37 μg As5+ g-1 on 100 ppb in concentration. High concentration of phosphate addition (2 and 5 μM) in seawater resulted in significant higher of growth values (p<0.05) than without phosphate addition treatment. All arsenate (As5+) and total arsenic of thalli under all treatments were significantly decrease (p<0.05) once thalli moved into arsenic-free environment. It suggested the methylation of arsenic was may due from arsenate (As5+) to become less toxic organic arsenic than excrete it to the outside of cell as detoxification mechanism.

Other Language Abstract

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Item Type: Thesis (Magister)
Identification Number: TES/577.627/VIO/s/2017/041706896
Uncontrolled Keywords: ARSENIC - TOXICOLOGY, ARSENIC - ENVIRONMENTAL ASPECTS, ARSENIC - BIOACCUMULATION, WATER - POLLUTION, MARINE ALGAE, MARINE ALGAE - TOXICOLOGY
Subjects: 500 Natural sciences and mathematics > 577 Ecology > 577.6 Aquatic ecology > 577.627 Water Pollution (Effect on natural ecology)
Divisions: S2 / S3 > Magister Bioteknologi Perikanan dan Kelautan, Fakultas Perikanan dan Ilmu Kelautan
Depositing User: Nur Cholis
URI: http://repository.ub.ac.id/id/eprint/1440
Text
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